Objective: To evaluate effects of long term storage of semen in liquid nitrogen on the motility, concentration, viability, and abnormality of frozen-thawed. Methods: A total of four Friesian Holstein bulls were used for this study. One hundred forty semen straws with produced during period from 2008 to 2013 and stored in the liquid nitrogen at the AI center were used in the research. The sample straw was divided into six groups;each group consist 20 semen straws. For group one all straw semen was produced on the 2013 with storage in liquid nitrogen as long as one year, the group 2, 3, 4, 5, and 6 were produced on the 2012, 2011, 2010, 2009 and 2008 with storage in liquid nitrogen as long as 2, 3, 4, 5, and 6 year, respectively. Results:The viability of thawed sperm was not significantly different decreased (P>0.05) between storage on the 1 year and storage on the 2 years. Whereas, the viability was significantly different (P<0.05) with storage on the 3, 4, 5 and 6 years. The motility of thawed sperm was not significantly different decreased (P>0.05) on the storage 1, 2 and 3 years. Whereas, the motility was significantly different (P<0.05) with storage on the 4, 5 and 6 years. The abnormality of thawed sperm was not significantly different increased (P>0.05) on the storage 1, 2 and 3 years. Whereas, the abnormality was significantly increased (P<0.05) with storage on the 4, 5 and 6 years. On the other hand the concentration of thawed sperm was not significantly different decreased (P>0.05) during storage in liquid nitrogen as long as six years. Conclusions:Based on the results in these experiments, it may be concluded that concentration sperm during one year storage in liquid nitrogen resulted in similar concentration storage as long as six years. However, the viability and motility sperm thawed storage in liquid nitrogen during six years was lower than storage on the 1 and 2 years.