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目的 探讨抗原致敏和激发后肺局部免疫反应的变化。方法 以抗原钥孔嘁血兰素(KLH)经气管内滴注致敏小鼠 ,2～ 4周后进行激发 ,期间分别收集支气管肺泡灌洗液 (BALF)和外周血 ,对肺、肺相关淋巴结 (LALN)和脾组织细胞悬液进行培养并收集上清液 ,以酶联免疫吸附试验(ELISA)测定总IgA、抗KLHIgA及白蛋白。结果 KLH致敏和激发均引起BALF中抗KLHIgA反应。抗原致敏和激发后BALF中抗KLHIgA/白蛋白比率明显高于血清 ;抗原致敏激发后肺和LALN细胞悬液在体外均可释放抗KLHIgA ,而脾细胞悬液仅释放低水平抗KLHIgA。结论 气管内滴注抗原致敏小鼠可诱导肺局部抗原特异IgA反应 ,抗原激发后反应加强。肺局部积聚的抗KLHIgA并非由血中渗漏而来 ,而是局部产生的结果。肺和LALN中的淋巴细胞是抗原特异IgA的主要来源。
Objective To investigate the changes of local immune response in lung after antigen sensitization and challenge. Methods The mice were sensitized by endotracheal instillation of antigen keyhole limpet hemocyanin (KLH), and were stimulated 2 to 4 weeks later. Bronchoalveolar lavage fluid (BALF) and peripheral blood were collected, Lymph node (LALN) and spleen tissue cell suspension were cultured and the supernatant was collected. Total IgA, anti-KLHIgA and albumin were determined by enzyme-linked immunosorbent assay (ELISA). Results Both KLH sensitization and challenge induced anti-KLHIgA responses in BALF. The ratio of anti-KLHIgA to albumin in BALF was significantly higher after antigen sensitization and challenge. Anti-KLHIgA could be released from lung and LALN cell suspension in vitro after antigen challenge, while spleen cell suspension only released low level of anti-KLHIgA. Conclusion Intratracheal instillation of antigen-sensitized mice can induce specific antigen-IgA response in lung, and the response is enhanced after antigen challenge. Local accumulation of anti-KLHIgA lung is not caused by leakage from the blood, but the result of the local. Lymphocytes in the lung and LALN are the major sources of antigen-specific IgA.