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目的 :研究多药耐药 (mnlti-drugresistance ,MDR)基因胎盘型谷胱甘肽 -S -转移酶 (GST -π)和DNA拓朴酶Ⅱ (TopoⅡ )以及MDR基因编码产物P -糖蛋白 (Pgp)、在咽喉部恶性黑色素瘤中的表达及其意义。方法 :应用链霉素亲生物素 -过氧化物酶标S -P法检测 2 8例咽喉部恶性黑色素瘤中Pgp、GST -π和TopoⅡ的表达 ,分析MDR基因及MDR基因编码产物阳性表达率与肿瘤主要临床病理特征的关系。结果 :2 8例标本中Pgp、GST -π和TopoⅡ的表达率分别为 35 .7%、5 7.1%和 4 6 .4 % ,相互间差异无显著性 (P >0 .0 5 )。Pgp、GST -π和TopoⅡ的表达与性别、年龄、肿瘤大小无明显相关 (P >0 .0 5 ) ,与AJC分级显著相关 (P <0 .0 5 )。结论 :Pgp、GST -π和TopoⅡ等多因素联合作用是咽喉部恶性黑色素瘤多药耐药的主要作用机理 ,其表达在化疗敏感性预测中具有必要性和可行性。
OBJECTIVE: To study the effects of multidrug resistance (MDR) gene placental glutathione S-transferase (GST-π) and TopoⅡ and the expression of the MDR gene P-glycoprotein Pgp) in malignant melanoma of the throat and its significance. Methods: The expression of Pgp, GST-π and TopoⅡ in 28 cases of throat malignant melanoma was detected by streptavidin-peroxidase-labeled S-P method. The positive rates of MDR gene and MDR gene were analyzed And the main clinical and pathological features of the tumor. Results: The expression rates of Pgp, GST-π and TopoⅡ in 28 samples were 35.7%, 51.1% and 46.4% respectively, with no significant difference between them (P> 0.05). The expression of Pgp, GST-π and TopoⅡwas not significantly correlated with gender, age, tumor size (P> 0.05), but significantly correlated with AJC classification (P <0.05). Conclusion: The combination of Pgp, GST-π and Topo Ⅱ is the main mechanism of multidrug resistance in malignant melanoma of the throat. The expression of Pgp, GST-π and TopoⅡ is necessary and feasible in the prediction of chemosensitivity.