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A hybrid learning method combining immune algorithm and least square method is proposed to design the radial basis function(RBF) networks. The immune algorithm based on information entropy is used to determine the structure and parameters of RBF nonlinear hidden layer, and weights of RBF linear output layer are computed with least square method. By introducing the diversity control and immune memory mechanism, the algorithm improves the efficiency and overcomes the immature problem in genetic algorithm. Computer simulations demonstrate that the RBF networks designed in this method have fast convergence speed with good performances.