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目的通过探讨Foxo1-KLF2-S1P1在MG患者胸腺的表达,分析其对胸腺T细胞输出的影响。方法取MG患者及对照组胸腺组织,提取总RNA,通过荧光定量RT-PCR检测Foxo1、KLF2、S1P1及CD62L、CCR7、CD69的表达;制作胸腺组织切片,通过免疫组化了解Foxo1、S1P1、CD62L、CD69、CCR7在胸腺组织的分布与表达。结果免疫组化显示Foxo1、S1P1、CCR7在MG患者及对照组胸腺都有表达,主要分布在髓质区,MG患者胸腺组织髓质区扩大,Foxo1、S1P1、CCR7表达显著高于对照组;荧光定量PCR结果显示MG胸腺组织Foxo1、KLF2、S1P1、CCR7、CD69的m RNA的水平表达显著增高(分别是5.36±0.728、1.43±2.71、6.1±1.033、5.0±0.932、4.97±1.112,与对照组相比P<0.05)。结论 MG患者Foxo1-KLF2-S1P1高表达可能是MG患者胸腺细胞的异常输出的原因。
Objective To investigate the expression of Foxo1-KLF2-S1P1 in the thymus of patients with MG and analyze its effect on the export of thymic T cells. Methods The total RNA was extracted from the thymus tissue of MG patients and control group. The expression of Foxo1, KLF2, S1P1, CD62L, CCR7 and CD69 was detected by real-time RT-PCR. Thymus tissue sections were obtained. Immunohistochemistry was used to detect Foxo1, S1P1, CD62L , CD69, CCR7 in thymic tissue distribution and expression. Results Immunohistochemistry showed that Foxo1, S1P1 and CCR7 were expressed in the thymus of MG patients and controls, mainly in the medulla, MG medulla in thymus, and the expressions of Foxo1, S1P1 and CCR7 were significantly higher than those in control Quantitative PCR results showed that m RNA levels of Foxo1, KLF2, S1P1, CCR7 and CD69 in MG thymus were significantly increased (5.36 ± 0.728, 1.43 ± 2.71, 6.1 ± 1.033, 5.0 ± 0.932 and 4.97 ± 1.112, respectively) Compared to P <0.05). Conclusion The high expression of Foxo1-KLF2-S1P1 in MG patients may be responsible for the abnormal output of thymocytes in MG patients.