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目的探讨癌基因MUC4在食管癌中的拷贝数变化及其意义。方法应用FISH方法检测MUC4基因在35例食管癌组织中的拷贝数变化,并与18例癌旁组织做对比研究。结果 MUC4基因在35例食管癌组织均出现高频率(100％)扩增,18例癌旁组织中仅5例出现低频率扩增(27.8％),两者之间差异极显著(P0.05)。 结论MUCA基因扩增可能与食管癌的发生关系密切。
Objective To investigate the change of copy number of oncogene MUC4 in esophageal cancer and its significance. Methods FISH was used to detect the copy number changes of MUC4 gene in 35 cases of esophageal cancer and compared with 18 cases of paracancerous tissues. Results MUC4 gene showed high frequency (100%) amplification in 35 cases of esophageal cancer tissues and low frequency amplification (27.8%) in only 5 cases of 18 cases of paracancerous tissues, the difference was significant (P <0 .01). MUC4 gene amplification frequency and esophageal cancer patients age, gender, tumor TNM stage, lymph node metastasis and the degree of differentiation was not related (P> 0.05). Conclusion MUCA gene amplification may be closely related to the occurrence of esophageal cancer.