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目的比较枸杞多糖与丹参酮对衰老小鼠皮肤的抗衰老作用。方法60只雌性昆明种小鼠,随机分为4组(n=15):正常对照组皮下注射生理盐水;其余3组每日颈背部皮下注射D-半乳糖[1000mg/(kg·d)]造成小鼠衰老模型,同时各组分别灌胃生理盐水(衰老模型组)、枸杞多糖[200mg/(kg·d)]、丹参酮[1500mg/(kg·d)]。42d后,测定小鼠背部皮肤组织匀浆中超氧化物歧化酶(SOD)活力、过氧化脂质代谢产物丙二醛(MDA)含量和皮肤羟脯氨酸(Hyp)含量。结果枸杞多糖可使衰老模型小鼠皮肤中MDA含量显著降低,较丹参酮组明显;但SOD活力显著升高,不及丹参酮组改变显著;枸杞多糖和丹参酮均可使羟脯氨酸含量升高,且效果相当。结论枸杞多糖与丹参酮都有显著的抗小鼠皮肤衰老作用,枸杞多糖清除皮肤中脂质过氧化代谢产物方面较丹参酮明显,而提高皮肤抗氧化酶活力则不及丹参酮显著,在增强成纤维细胞活性方面两者效果相当。
Objective To compare the anti-aging effects of lycium barbarum polysaccharides and tanshinone on aging skin. Methods Sixty female Kunming mice were randomly divided into four groups (n=15): Normal control group received subcutaneous injection of normal saline; the other three groups received daily subcutaneous injection of D-galactose [1000 mg/(kg·d)]. The aging model was induced in mice. At the same time, each group was given normal saline (aging model group), lycium barbarum polysaccharide [200 mg/(kg·d)] and tanshinone [1500 mg/(kg·d)]. After 42 days, superoxide dismutase (SOD) activity, lipid peroxidation product malondialdehyde (MDA) content and skin hydroxyproline (Hyp) content in the skin homogenate of mice dorsal surface were measured. RESULTS: Compared with the tanshinone group, wolfberry polysaccharides significantly decreased the content of MDA in the skin of the aging model mice, but the SOD activity increased significantly, which was less than that in the tanshinone group. Both wolfberry polysaccharides and tanshinones increased the hydroxyproline content. The effect is quite similar. Conclusion Both LBP and tanshinone have significant anti-aging effects on skin in mice. LPS can clear the lipid peroxidation metabolites in the skin more than tanshinone. However, the increase of skin antioxidant enzyme activity is less than that of tanshinone, and it can enhance the activity of fibroblasts. In terms of both, the effect is quite similar.