论文部分内容阅读
目的:观察姜黄素对神经病理性痛大鼠痛行为及脊髓背角的p-ERK、p-CREB表达的影响。方法:采用大鼠坐骨神经慢性压迫性损伤(CCI)模型。雄性SD大鼠108只,随机分为实验对照(Con)组、假手术(Sham组)、溶剂对照(SC)组、姜黄素(Cur30、Cur100、Cur300)组。姜黄素组行CCI术,每组每天分别腹腔注射姜黄素30、100、300mg/kg,持续14天。各组于术前2天和术后1、3、5、7、10、14天测定机械痛阈(MWT)和热痛阈(TWL)。在术后3、7、14天处死大鼠(n=6),分别制备大鼠L4-5脊髓的石蜡标本,用免疫组织化学方法测定p-ERK、p-CREB在脊髓背角神经元的动态变化。结果:Con组大鼠MWT和TWL呈进行性下降,在术后3天降至最低(MWT为15.3±3,TWL为4.6±1.0),术侧脊髓背角浅层p-ERK、p-CREB阳性神经元明显增多;Cur组大鼠在术后MWT和TWL亦进行性下降,在术后3天降至最低(Cur100组的MWT为22.6±4,TWL为5.6±1.1),与Con组相比,每组每个时间点的MWL、TWL均明显升高(P<0.01);术侧的脊髓背角浅层p-ERK、p-CREB阳性神经元的表达下降,每组每个时间点表达均低于Con组(P<0.01)。结论:在本实验中,姜黄素能减轻神经病理性痛,其机制可能与降低p-ERK、p-CREB阳性神经元的表达有关。
OBJECTIVE: To observe the effects of curcumin on the pain behavior of rats with neuropathic pain and the expression of p-ERK and p-CREB in spinal dorsal horn. METHODS: A chronic constriction injury (CCI) model of rat sciatic nerve was used. 108 male SD rats were randomly divided into control group (Con), sham operation (Sham group), solvent control (SC) group, and curcumin (Cur30, Cur100, Cur300) group. The curcumin group underwent CCI surgery. Each group received intraperitoneal injection of curcumin 30, 100, 300 mg/kg daily for 14 days. The mechanical pain threshold (MWT) and thermal pain threshold (TWL) were measured on the 2nd day before surgery and on 1, 3, 5, 7, 10, and 14 days after surgery. Rats (n=6) were sacrificed 3, 7 and 14 days after surgery. Rat L4-5 spinal cord paraffin specimens were prepared. Immunohistochemical method was used to determine the neurons of p-ERK and p-CREB in spinal dorsal horn neurons. Dynamic changes. RESULTS: MWT and TWL were progressively decreased in the Con group and decreased to a minimum at 3 days postoperatively (MWT was 15.3±3, TWL was 4.6±1.0). Shallow p-ERK and p-CREB in the spinal cord of the operated side Positive neurons increased significantly; in the Cur group rats, MWT and TWL also decreased progressively, and decreased to a minimum at 3 days after operation (MWT of Cur100 group was 22.6±4, TWL was 5.6±1.1), compared with Con group. The MWL and TWL at each time point in each group were significantly increased (P<0.01); the expression of p-ERK and p-CREB positive neurons in the spinal cord at the operative side decreased, with each group of time points. The expression was lower in the Con group (P<0.01). Conclusion: In this experiment, curcumin can reduce neuropathic pain, and its mechanism may be related to the decrease of p-ERK and p-CREB positive neurons.