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目的观察眼镜蛇毒因子(CVF)抑制补体激活对创伤失血性休克大鼠肠道的影响。方法雄性SD大鼠建立创伤失血性休克模型,随机分成对照组和CVF组,根据观察时间点的不同各组再分为休克前、复苏后1、6、24 h时相组。结果对照组大鼠复苏后1 h血总补体活性(CH_(50))水平迅速下降,血内毒素(LPS)浓度、肠黏膜损伤评分均明显升高,随后2个时间点快速恢复至休克前水平;二胺氧化酶(DAO)活性在随后1 h和6 h时相明显升高,然后快速下降。CVF组大鼠除CH_(50)水平始终<5%,其余各指标复苏后1 h仅略升高,其复苏后各时相组均较对照组相应时相组明显降低。结论在创伤失血性休克中使用补体抑制剂可明显减轻补体激活导致的肠道损伤及肠屏障破坏,减少内毒素移位的发生,明显降低血浆内毒素含量。
Objective To observe the effect of cobra venom factor (CVF) on complement activation in the intestinal tract of traumatic hemorrhagic shock rats. Methods The traumatic hemorrhagic shock model was established in male Sprague-Dawley rats and randomly divided into control group and CVF group. According to different observation time points, each group was divided into pre-shock group, 1, 6 and 24 h group. Results The level of total complement activity (CH_(50)) decreased rapidly in the control group 1 hour after resuscitation. The blood endotoxin (LPS) concentration and the score of intestinal mucosal injury were significantly increased, and then quickly recovered to shock before 2 hours later. The level of diamine oxidase (DAO) activity increased significantly at 1 h and 6 h, and then decreased rapidly. The levels of CH_(50) in CVF rats were always <5%, and the other indicators only increased slightly at 1 h after resuscitation. After the resuscitation, the time of each phase group was significantly lower than that of the control group. Conclusion The use of complement inhibitors in traumatic hemorrhagic shock can significantly reduce intestinal damage and intestinal barrier damage caused by complement activation, reduce the occurrence of endotoxin translocation, and significantly reduce plasma endotoxin content.