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[目的]探讨人β防御素-1联合冬凌草甲素对膀胱癌T24细胞的生长抑制作用及机制。[方法]以不同浓度的β防御素-1、冬凌草甲素单独或联合作用于体外培养的T24细胞,MTT法检测细胞生长抑制率,应用Hoechst 33258荧光染色法以流式细胞仪观察细胞凋亡,显微镜下观察细胞的形态学变化。[结果]人β防御素-1及冬凌草甲素均可抑制T24细胞的生长,引起T24细胞溶解坏死,并呈现明显的浓度—效应与时间—效应关系。[结论]人β防御素-1、冬凌草甲素均对膀胱癌具有明显的体外抗肿瘤作用,两者联合能对膀胱癌细胞起协同杀伤作用,杀伤机制以细胞毒性作用为主。
[Objective] To investigate the inhibitory effect of human beta-defensin-1 combined with oridonin on the growth of bladder cancer T24 cells and its mechanism. [Method] T24 cells cultured in vitro were treated with different concentrations of β-defensin-1 and oridrin A. Cell growth inhibition rate was determined by MTT assay. The cells were observed by flow cytometry with Hoechst 33258 fluorescent staining Apoptosis, microscopic observation of the morphological changes of cells. [Results] Both human β-defensin-1 and oridonin inhibited the growth of T24 cells, caused the lytic necrosis of T24 cells, and showed a significant concentration-effect and time-effect relationship. [Conclusion] Human β-defensin-1 and oridonin all have obvious anti-tumor effects on bladder cancer in vitro. The combination of the two can have a synergistic killing effect on bladder cancer cells. The cytotoxicity is the main mechanism of cytotoxicity.