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目的探究白色念珠菌(C.albicans)经口感染ICR小鼠建立系统性感染模型,观察C.albicans经黏膜感染后在小鼠体内组织增殖及分布规律。方法 46只ICR雄性小鼠随机分为模型组A(n=20)、模型组B(n=20)、对照组(n=6)。模型组采用棉签法口腔接种C.albicans(7×106cfu/m L),对照组同方法接种等容积生理盐水。模型组A小鼠用于临床、生存、剖检观察试验;模型组B接种后第3、5、7天随机解剖5只小鼠用于组织载菌量和病理检验(对照组每个时间点解剖2只),活菌平板计数法检测小鼠组织载菌量,光镜观察小鼠舌、胃、肝、肾病理组织学变化。结果模型组小鼠接种后第3天舌面出现明显白斑并随时间加重引起死亡,第5天小鼠死亡率超过50%,第7天死亡率达100%,并能从舌(87.5%)、胃(87.5%)及内脏组织肝(54.5%)、肾(50.5%)、肺(20%)和心脏(4%)中分离到C.albicans,显微镜观察在舌、食道、胃、肝、肾存在菌丝的增殖,对照组未见C.albicans生长,提示模型组小鼠因C.albicans黏膜感染引起小鼠播散性的系统性感染。结论 C.albicans可在一定条件下突破黏膜免疫屏障引起小鼠机会性系统性感染,从而加重感染死亡。
Objective To investigate the systemic infection model of C. albicans infected with ICR mice by oral administration and observe the proliferation and distribution of C.albicans in mice after mucosal infection. Methods 46 ICR male mice were randomly divided into model group A (n = 20), model group B (n = 20) and control group (n = 6). The model group was swallowed C.albicans (7 × 106cfu / m L) by cotton swabs, and the control group was inoculated with the same volume of normal saline. Model group A mice were used for clinical, survival and necropsy observation; model group B were randomly dissected on the 3rd, 5th, 7th day after inoculation for 5 mice in order to inoculate the bacteria and pathological examination (control group at each time point Anatomy 2), viable plate count method was used to detect the amount of bacteria in mice tissues. Pathological changes of tongue, stomach, liver and kidney were observed by light microscope. Results On the 3rd day after inoculation, the mice in the model group showed obvious leukoplakia and died with the increase of time. On the 5th day, the mortality of the mice was over 50%, the mortality rate was 100% on the 7th day, and from the tongue (87.5%), C.albicans was isolated from the stomach (87.5%) and visceral liver (54.5%), kidney (50.5%), lung (20%) and heart (4% There was mycelial proliferation in the kidney, but no growth of C.albicans in the control group, which suggested that mice infected by C.albicans mucosa in the model group caused systemic infection in mice. Conclusion C. albicans can break through the mucosal immune barrier under certain conditions, causing opportunistic systemic infections in mice, thus aggravating the infection death.