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目的 检测正常成年男性附睾中不同部位的精子指标 ,为男性附睾精子功能的研究提供实验资料。方法 将附睾切断分成附睾头、附睾尾和附睾体 ,分别纵向剖开 ,置于含有 10 %小牛血清的F10 溶液中 ,37℃孵育 30min ,孵育液作精子常规分析、低渗肿胀试验 (HOS)、精子 -宫颈粘液穿透试验。结果 附睾不同部位精子的形态和功能存在明显差异 ,尾部和体部精子的活率、活动力、HOS试验和CM穿透试验结果明显高于头部精子 ,差异有显著性 (P <0 .0 5或 0 .0 1) ,以尾部精子的各项指标最高 ,与正常排出体外的精子无显著差异 (P >0 .0 5 )。头部附睾未成熟精子、畸形精子比例明显高于尾部和体部附睾的精子 (P <0 .0 1和 0 .0 5 )。结论 附睾精子可应用于辅助生育技术 ,作为治疗梗阻性无精子症的最好选择。
Objective To detect sperm parameters of different parts of normal adult male epididymis and provide experimental data for the study of male epididymal sperm function. Methods The epididymis was divided into epididymis, epididymis and epididymis, respectively, longitudinally sectioned and placed in F10 solution containing 10% fetal bovine serum for 30 min at 37 ° C. The incubation solution was used for sperm routine analysis, hypotonic swelling test (HOS ), Sperm - cervical mucus breakthrough test. Results The morphology and function of spermatozoa in different parts of epididymis were significantly different. The viability, viability, HOS test and CM penetration test of tail and body sperm were significantly higher than those of head sperm (P <0. 0 5 or 0 .0 1). The sperm in the tail had the highest indexes, but no significant difference (P> 0.05) with the normal sperm excreted. The proportion of immature sperm and deformed sperm in the head epididymis was significantly higher than that in the tail and body epididymis (P <0.01 and 0.05). Conclusion Epididymal sperm can be used in assisted reproductive technology as the best choice for the treatment of obstructive azoospermia.