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以‘红阳’猕猴桃雌株幼叶为外植体,直接诱导产生不定芽,并对不定芽增殖以及生根体系进行优化,建立了高频再生体系。结果表明,在MS+3.0 mg/L BA+1.0 mg/L NAA培养基中,不定芽诱导率达100%,平均出芽数达18.67个/叶盘;在MS+2.0 mg/L BA+1.0 mg/L NAA+0.1 mg/L GA3培养基中,增殖率达100%,1~6代不定芽平均繁殖系数达8.63;不定芽在1/2 MS+0.8 mg/L IBA培养基中培养15 d,然后继代至含1/2 MS液体培养基的珍珠岩中培养15 d,生根率达100%,且其根系发育良好;98株试管苗移栽到土壤盆钵中,成活95株,成活率达96.94%。本试验成功建立了‘红阳’猕猴桃叶片高频直接再生体系,该体系诱导产生不定芽周期短,出芽率高,不定芽增殖系数大,生根率高且根系发达,为‘红阳’猕猴桃试管苗的工厂化生产和遗传转化奠定了基础。
The young leaves of ’Hongyang’ kiwifruit female plant were used as explants to induce adventitious buds directly and to optimize the proliferation and rooting system of adventitious buds. A high-frequency regeneration system was established. The results showed that the induction rate of adventitious buds was 100% and the average number of buds was 18.67 / leaf discs in MS + 3.0 mg / L BA + 1.0 mg / L NAA medium. Under MS + 2.0 mg / L BA + 1.0 mg / L NAA + 0.1 mg / L GA3 medium, the proliferation rate reached 100%, the average propagation coefficient of adventitious buds from 1 to 6 generations reached 8.63; Adventitious buds were cultured in 1/2 MS + 0.8 mg / L IBA medium for 15 days , And then subcultured perlite containing 1/2 MS liquid medium for 15 days. The rooting rate reached 100%, and its root system developed well. 98 test-tube seedlings were transplanted into the soil pot and survived 95 Rate of 96.94%. In this study, we successfully established a high-frequency direct regeneration system of ’Hongyang’ kiwifruit leaves. The system induced short adventitious buds, high germination rate, large multiplication coefficient of adventitious buds, high rooting rate and developed root system. Seedling factory production and genetic transformation laid the foundation.