全基因组扩增(whole genome amplification,WGA)技术是一种对全部基因组序列进行非选择性扩增的技术。对微量模板DNA的检验,一直是法医物证学特别关注和急需解决的难题之一。近年来,对WGA技术的的研究发展日渐深入,研究成果有望应用于法医DNA检验中各类微量检材的检验。本文综述了WGA技术在法医物证学中的研究进展和应用前景,为法医物证工作者提供参考。 Whole genome amplification (WGA) technology is a technique for nonselective amplification of the entire genome sequence. The test of trace template DNA has always been one of the most important problems to be solved in forensic evidence. In recent years, the research and development of WGA technology is becoming more and more in depth. The research results are expected to be applied to the inspection of various kinds of micro-samples in forensic DNA testing. This article reviews the research progress and application prospects of WGA in forensic evidence-based science, and provides a reference for forensic evidence practitioners.