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本研究旨在探讨可溶性endoglin(soluble endoglin,sEng)对人早孕细胞滋养细胞活力和浸润功能的影响。采用胰蛋白酶-DNase消化法培养人早孕期(孕6～8周)细胞滋养细胞,传代后待细胞长满至70%～80%,分别加入没有添加(对照组)和添加sEng(10μg/L)的细胞培养液培养24h;Transwell技术检测细胞滋养细胞的浸润功能;RT-PCR法检测细胞滋养细胞基质金属蛋白酶2(matrix metalloproteinase-2,MMP-2)、基质金属蛋白酶9(MMP-9)mRNA的表达;Western blot方法分别检测细胞滋养细胞MMP-2、MMP-9蛋白的表达。结果显示,sEng组细胞滋养细胞浸润能力低于对照组。与对照组比较,sEng组细胞滋养细胞MMP-2和MMP-9 mRNA及蛋白的表达明显下降(P<0.05)。以上结果提示,sEng可能通过调节人早孕细胞滋养细胞中MMP-2、MMP-9的表达而影响细胞的浸润能力,从而参与子痫前期的发生。
This study aimed to investigate the effect of soluble endoglin (sEng) on the cytotrophoblast activity and infiltration in human early pregnancy. Trypsin-DNase digestion culture of human early pregnancy (gestational 6 to 8 weeks) cytotrophoblast cells were passaged until the cells grew to 70% to 80% were added without added (control group) and added sEng (10μg / L ) Cells were cultured for 24 h. Transwell technique was used to detect the infiltration of cytotrophoblasts. RT-PCR was used to detect the expression of matrix metalloproteinase-2 (MMP-2), matrix metalloproteinase 9 (MMP-9) The mRNA expression of MMP-2 and MMP-9 were detected by Western blot. The results showed that sEng group cytotrophoblast infiltration capacity was lower than the control group. Compared with the control group, the mRNA and protein expressions of MMP-2 and MMP-9 in sEng group were significantly decreased (P <0.05). The above results suggest that sEng may participate in the development of preeclampsia by regulating the expression of MMP-2 and MMP-9 in human gestational trophoblastic cells.