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目的:探讨毒素清颗粒对内毒素肺损伤家兔小肠组织巨噬细胞、肿瘤坏死因子-α(TNF-α)、细胞间黏附因子-1(ICAM-1)和血管间黏附因子-1(VCAM-1)表达的影响。方法:静脉注射脂多糖建立家兔肺损伤模型。大耳白兔36只,随机分为空白对照组、模型组、毒素清大中小剂量组及双黄连组。免疫组织化学法测定各组小肠组织巨噬细胞、TNF-α、ICAM-1和VCAM-1的表达。结果:与对照组比,模型组小肠组织巨噬细胞、TNF-α、ICAM-1、VCAM-1表达显著增强(P<0.01);与模型组比,毒素清各剂量组和双黄连组巨噬细胞、TNF-α表达明显减弱(P<0.01),毒素清各剂量组肠组织中ICAM-1阳性单位PU值和毒素清大、中剂量组VCAM-1阳性单位PU值明显减低(P<0.01,P<0.05);与双黄连组比,毒素清大剂量组巨噬细胞、TNF-α、ICAM-1、VCAM-1阳性单位PU值明显减低(P<0.05,P<0.01)。结论:模型组小肠组织巨噬细胞、TNF-α、ICAM-1、VCAM-1表达显著增强,毒素清能显著抑制巨噬细胞、TNF-α、ICAM-1、VCAM-1表达,减轻炎症反应。
Objective:To investigate the effect of Duoxinqing Granules on macrophage, tumor necrosis factor-α (TNF-α), intercellular adhesion molecule-1 (ICAM-1) and intervascular adhesion factor-1 (VCAM) in small intestine of rabbits with endotoxin-induced lung injury. -1) Influence of expression. METHODS: Rabbit models of lung injury were established by intravenous injection of lipopolysaccharide. Thirty-six large white rabbits were randomly divided into blank control group, model group, toxin Qingda medium and small dose group and Shuanghuanglian group. The expression of macrophages, TNF-α, ICAM-1, and VCAM-1 in the small intestine of each group was determined by immunohistochemistry. Results: Compared with the control group, the expression of macrophage, TNF-α, ICAM-1 and VCAM-1 in the small intestine tissue of the model group was significantly increased (P<0.01). Compared with the model group, the toxin clearing group and the Shuanghuanglian group were significantly different. The expression of TNF-α and phagocytes was significantly attenuated (P<0.01). The ICAM-1 positive unit PU value in the intestines and the VCAM-1 positive unit PU values in the toxin Qingda and middle dose groups were significantly reduced in the toxin clearing groups (P<0.01). 0.01, P<0.05); compared with Shuanghuanglian group, the macrophage, TNF-α, ICAM-1, VCAM-1 positive unit PU value of the large dose of toxin clear group significantly decreased (P<0.05, P<0.01). Conclusion: The expression of macrophage, TNF-α, ICAM-1 and VCAM-1 in the small intestine of the model group is significantly enhanced. Toxin clearing significantly inhibits the expression of macrophage, TNF-α, ICAM-1 and VCAM-1 and reduces the inflammatory response. .