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目的 :研究胸苷激酶 (TK)自杀基因疗法对人前列腺癌细胞的杀伤作用和旁观者效应 ,探讨其治疗前列腺癌的可行性。方法 :采用形态学观察、活细胞拒染法及MTT法检测单纯疱疹病毒胸苷激酶 (HSV TK) /羟基无环鸟苷 (GCV)系统对前列腺癌细胞的杀伤作用及旁观者效应。结果 :GCV对转染TK基因前列腺癌细胞的杀伤作用呈剂量及时间依赖关系 (P <0 .0 5～ 0 .0 1) ;但其旁观者效应较弱 ,TK基因阳性细胞比例约为 10 %的混合细胞 ,经 10 μmol/L和 10 0 μmol/L的GCV处理 72h后 ,仅有16.15 %± 1.64 %和 2 3 .46%± 3 .2 1%的细胞被杀灭。结论 :HSV TK/GCV系统对前列腺癌有杀伤作用 ,但由于其旁观者效应不够强大 ,不能期望通过单纯的TK基因治疗达到前列腺癌良好的治疗效果
Objective: To study the killing effect and bystander effect of thymidine kinase (TK) suicide gene therapy on human prostate cancer cells and explore the feasibility of its treatment for prostate cancer. Methods: The killing effect and bystander effect of herpes simplex virus thymidine kinase (HSV TK) / hydroxycyclic guanosine monophosphate (GCV) system on prostate cancer cells were detected by morphological observation, viable cell staining and MTT assay. Results: The killing effect of GCV on TK gene transfected prostate cancer cells in a dose-and time-dependent manner (P <0.05-0.01), but the bystander effect was weaker and the percentage of TK-positive cells was about 10 % Of mixed cells, only 16.15% ± 1.64% and 23.46% ± 3.21% of the cells were killed after being treated with GCV at 10 μmol / L and 100 μmol / L for 72 h. CONCLUSIONS: The HSV TK / GCV system has a killing effect on prostate cancer, but due to its bystander effect is not strong enough to expect a good therapeutic effect of prostate cancer by pure TK gene therapy