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目的:观察大豆异黄酮对大鼠血管平滑肌细胞增殖和表型转化的影响。方法:采用酶消化法原代培养大鼠血管平滑肌细胞,传至第3～6代,加入含不同浓度(0、0.1、0.2、0.4、0.8μmol/L)大豆异黄酮的DMEM培养基作用24 h,采用MTT法检测大鼠血管平滑肌细胞增殖率,采用RT-PCR法检测平滑肌22α(SM22α)mRNA、骨桥蛋白mRNA的表达,采用Western blot法检测SM22α、骨桥蛋白的表达。结果:0.1～0.8μmol/L的大豆异黄酮随浓度增加其抑制大鼠血管平滑肌细胞增殖的作用增强(P<0.05);SM22αmRNA及SM22α蛋白表达增加,骨桥蛋白mRNA及骨桥蛋白表达降低,各组间有明显差异(P<0.05)。结论:大豆异黄酮抑制大鼠血管平滑肌细胞增殖,同时使大鼠血管平滑肌细胞从合成表型向收缩表型转化。
Objective: To observe the effect of soybean isoflavone on the proliferation and phenotype transformation of rat vascular smooth muscle cells. METHODS: Primary rat vascular smooth muscle cells were cultured by enzymatic digestion and passed to the third to sixth passages. DMEM medium containing different concentrations (0, 0.1, 0.2, 0.4, and 0.8 μmol/L) of soy isoflavones was added. h, The proliferation rate of rat vascular smooth muscle cells was detected by MTT assay. The expression of smooth muscle 22α (SM22α) mRNA and osteopontin mRNA was detected by RT-PCR. The expression of SM22α and osteopontin was detected by Western blot. RESULTS: 0.1-0.8 μmol/L soybean isoflavone increased the inhibitory effect on the proliferation of rat vascular smooth muscle cells with increasing concentrations (P<0.05); the expression of SM22α mRNA and SM22α protein increased, and the expression of osteopontin mRNA and osteopontin decreased. There was a significant difference between the groups (P<0.05). CONCLUSION: Soybean isoflavones inhibited the proliferation of rat vascular smooth muscle cells and induced the conversion of rat vascular smooth muscle cells from a synthetic phenotype to a contractile phenotype.