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目的:通过体外药效评价实验研究荆芥总黄酮对人结肠癌细胞Caco-2的影响。方法:不同浓度荆芥总黄酮作用Caco-2细胞24、48、72 h,检测其对Caco-2细胞增殖的影响,荆芥总黄酮配成低(0.3 mg/m L)、中(0.9 mg/m L)、高(2.5 mg/m L)浓度,作用Caco-2细胞48 h后,流式细胞仪分析细胞周期及凋亡情况。结果:随着药物浓度的升高及作用时间的延长,其对Caco-2细胞的增殖抑制率呈上升趋势。与空白对照组比较,荆芥总黄酮各组凋亡细胞比例显著升高,细胞分裂周期的各时相所占的比例也发生了改变,其中G0/G1期、S期降低比例显著升高,G2/M期比例显著降低。结论:荆芥总黄酮对Caco-2细胞具有一定的增殖抑制作用。可通过诱导细胞凋亡、抑制肠癌细胞从G0/G1期向S期转化,’加S期细胞比例以延长肿瘤细胞的分裂周期,从而抑制肿瘤细胞的增殖。
OBJECTIVE: To study the effect of total flavonoids of Nepeta on Caco-2 in human colon cancer cells in vitro. Methods: Caco-2 cells were treated with different concentrations of total flavonoids of Nepeta medullae for 24, 48, and 72 h, and their effects on the proliferation of Caco-2 cells were examined. / m L) and high concentration (2.5 mg / m L) for 48 h, the cell cycle and the apoptosis of Caco-2 cells were analyzed by flow cytometry. Results: The inhibitory rate of Caco-2 cells was increased with the increase of drug concentration and the prolongation of action time. Compared with the blank control group, the proportion of apoptotic cells in each group of Nepeta total flavonoids was significantly increased, and the proportion of each phase of the cell division cycle also changed. The proportion of G0 / G1 phase and S phase decreased significantly, G2 / M phase ratio decreased significantly. Conclusion: The total flavone of Nepeta has a certain inhibitory effect on Caco-2 cells. Through the induction of apoptosis, inhibition of colon cancer cells from G0 / G1 to S phase transformation, ’plus S phase cells to extend the proportion of cells in the division cycle, thereby inhibiting tumor cell proliferation.