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建立乙肝表面抗原胶体金免疫层析测定法，用于乙肝病毒感染检测。采用亲和力强、特异性高的自制抗─—HBs单克隆抗体及肢体金标记和硝酸纤维膜包被技术，制备乙肝表面抗原胶体金免疫层析快速测定试条，并进行了初步临床应用。该法的灵敏度为5ng／mL，与目前广泛使用的ELISA法对照检测513份血清标本，符合率为99．2％。该法快速、准确、特异、灵敏、简便，基本符合临床应用要求，可用于乙肝表面抗原的检测。
The establishment of hepatitis B surface antigen colloidal gold immunochromatographic assay for the detection of hepatitis B virus infection. The high affinity and high specificity of self-made anti-HBs monoclonal antibody and limb gold labeling and nitrocellulose membrane coating technology to prepare hepatitis B surface antigen colloidal gold immunochromatographic rapid determination of the test strips and preliminary clinical application. The sensitivity of this method is 5 ng / mL. Compared with the currently widely used ELISA method, 513 serum samples were tested, the coincidence rate was 99.2%. The method is rapid, accurate, specific, sensitive and simple, and basically meets the requirements of clinical application and can be used for the detection of hepatitis B surface antigen.