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本文采用PCR和直接序列分析以及原代细胞培养方法研究了32例垂体生长激素(GH)腺瘤的生化特征,发现21例gsp癌基因阴性肿瘤中大多对Octreotide无反应,而11例gsp癌基因阳性肿瘤中就有9例对Octreotide敏感。推测其机理可能与Octreotide通过GH释放抑制因子受体,进而GTP调节蛋白而负反馈调节腺苷酸环化酶活性所致。提示gsp癌基因可作为评价GH释放抑制因子类似物治疗GH腺瘤反应性的分子生物学指标。
In this study, the biochemical characteristics of pituitary growth hormone (GH) adenomas were studied using PCR and direct sequence analysis and primary cell culture methods. It was found that 21 of the gsp oncogene negative tumors were mostly unresponsive to Octreotide, while 11 cases were gsp oncogenes. Nine of the positive tumors were sensitive to Octreotide. It is speculated that the mechanism may be caused by negative feedback regulation of adenylate cyclase activity by Octreotide through the GH release inhibitor factor receptor, and then the GTP regulatory protein. It is suggested that the gsp oncogene can be used as a molecular biological indicator for evaluating the reactivity of GH release inhibitor analogs for the treatment of GH adenomas.