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本文建立了以流动注射停流技术测定纤维蛋白产品中曲酸的方法.借助于曲酸还原2,6-二氯酚钠(DCIP)产生的褪色反应,表观一级反应速率常数是曲酸初始浓度的 K 倍.通过停流时间观察 DCIP 颜色的变化,即通过第二峰高确定曲酸的含量.使用瑞典 FIAstar 5020分析仪和模式4,在设定的条件下(T_1=11秒,T_2=15秒,T_(inj)=10秒),线性范围为1～10mM.除抗坏血酸之外,许多有机酸和糖类不干扰测定。方法适用于纤维蛋白和饲料中曲酸的分析.
In this paper, a method for the determination of kojic acid in fibrin products by flow injection stopped-flow was established.According to the fading reaction of kojic acid-reduced sodium 2,6-dichlorophenol (DCIP), the apparent first-order reaction rate constant is kojic acid K times the initial concentration.ColorIPCD color change was observed by the stop flow time, that is, the content of kojic acid was determined by the second peak height.Using the Swedish FIAstar 5020 analyzer and mode 4, under the set conditions (T_1 = 11 seconds, T 2 = 15 seconds, T inj = 10 seconds) with a linear range of 1 to 10 mM Many organic acids and carbohydrates, except ascorbic acid, do not interfere with the assay. The method is suitable for the analysis of kojic acid in fibrin and feed.