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目的:探讨子宫内膜癌细胞在受到过氧化氢刺激时热休克因子1(Heat Shock Factor 1,HSF1)表达的变化,以及HSF1对肿瘤细胞抗凋亡能力的影响。方法:选择子宫内膜癌的Ishikawa、HEC-1-B及RL95-2三株细胞。分别测定细胞中HSF1基因转录以及翻译表达水平。给予细胞不同浓度的H2O2刺激后,检测细胞内HSF1的mRNA表达变化并且统计受刺激后细胞受抑制情况,观察细胞存活和生长与HSF1含量的关系。结果:三株细胞中HSF1mRNA和蛋白表达的基水平不同;在受到H2O2刺激后,细胞内HSF1表达有不同程度的升高;Ishikawa、HEC-1-B细胞分别在受到较高浓度的H2O2刺激后,细胞存活率出现明显下降;而RL95-2细胞在受到相对低浓度的H2O2刺激后,细胞存活率即出现明显降低。结论:一定范围内浓度的H2O2刺激能够上调子宫内膜癌细胞中HSF1在转录以及蛋白水平的表达,而过高的浓度会使细胞中HSF1表达减少,对于不同细胞来说H2O2刺激的适宜浓度不同。而能使细胞增殖与增长发生明显变化的H2O2浓度与细胞内HSF1表达水平相关。
Objective: To investigate the changes of heat shock factor 1 (HSF1) expression in endometrial carcinoma cells stimulated by hydrogen peroxide and the effect of HSF1 on the anti-apoptotic ability of tumor cells. Methods: Three strains of Ishikawa, HEC-1-B and RL95-2 were selected for endometrial cancer. The HSF1 gene transcription and translational expression levels in the cells were determined respectively. The cells were stimulated with different concentrations of H2O2 to detect the change of HSF1 mRNA expression in the cells, and the inhibition of the cells after stimulation was measured. The relationship between the cell survival and growth and the content of HSF1 was observed. Results: The expression of HSF1 mRNA and protein in the three cell lines differed at the basal level. After stimulated by H2O2, the expression of HSF1 increased to some extent. After Ishikawa and HEC-1-B cells were stimulated by H2O2 at higher concentrations, , Cell survival rate decreased significantly; while RL95-2 cells under relatively low concentrations of H2O2 stimulation, the cell survival rate that was significantly reduced. Conclusion: H2O2 stimulation in a certain range can increase the expression of HSF1 in the endometrial carcinoma cells at transcription and protein level, while the high concentration of HSF1 can decrease the expression of HSF1 in cells, and the suitable concentrations of H2O2 stimulation for different cells are different . The concentration of H2O2, which can significantly change the cell proliferation and growth, correlated with the expression level of HSF1 in cells.