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目的:建立兔脑垂体注射液中多肽的测定方法。方法:采用总蛋白试剂盒(TP)双缩脲比色法在540 nm处测定含量,并做回收率对照试验。结果:平均回收率为97.5％,RSD为1.6％。结论:该方法简便、快速、准确可靠。
Objective: To establish a method for determination of polypeptide in rabbit pituitary injection. Methods: Total protein kit (TP) biuret colorimetric assay at 540 nm, and the recovery rate control test. Results: The average recovery was 97.5% with a RSD of 1.6%. Conclusion: The method is simple, rapid, accurate and reliable.