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The 9-octadecanoic acid-hexadecanoic acid-tetrahydrofuran-3,4-diyl ester from neem oil was investigated for antibacterial activity against three bacterial strains viz.,Staphylococcus aureus ATCC No.25923,Escherichia coli ATCC No.44102 and Salmonella sp.ATCC No.50 041 in vitro.The minimum inhibitory concentration(MIC) and minimum bactericidal concentration(MBC) values of the 9-octadecanoic acid-hexadecanoic acid-tetrahydrofuran-3,4-diyl ester were determined by using the broth microdilution dilution(BMD) method at different concentrations ranging from 20 to 0.625 mg mL-1.Its time-inhibition curve against E.coli was also tested and showed that the MIC values for the bacterial strains S.aureus,E.coli and Salmonella sp.were 20,5 and 10 mg mL-1,respectively.Its MBC values were 20,20 and 10 mg mL-1,respectively.The antibacterial activity of 9-octadecanoic acid-hexadecanoic acid-tetrahydrofuran-3,4-diyl ester against three strain tested showed the relationship with time and concentration.
The 9-octadecanoic acid-hexadecanoic acid-tetrahydrofuran-3,4-diyl ester from neem oil was investigated for antibacterial activity against three bacterial strains viz., Staphylococcus aureus ATCC No. 25923, Escherichia coli ATCC No. 44102 and Salmonella sp. ATCC No.50 041 in vitro. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) values of the 9-octadecanoic acid-hexadecanoic acid-tetrahydrofuran- 3,4- diyl ester were determined by using the broth microdilution dilution ) method at different concentrations ranging from 20 to 0.625 mg mL-1.Its time-inhibition curve against E. coli was also tested and showed that the MIC values for the bacterial strain S. aureus, E. coli and Salmonella sp. were 20 , 5 and 10 mg mL-1, respectively.Its MBC values were 20,20 and 10 mg mL-1, respectively. The antibacterial activity of 9-octadecanoic acid-hexadecanoic acid-tetrahydrofuran-3,4-diyl ester against three strains demonstrated showed the relationship with time and concentration.