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胰岛素样生长因子Ⅱ(IGF-Ⅱ)是由67个氨基酸组成的多肽,主要经肝脏合成和分泌。有研究表明,肝病时血清中IGF-Ⅰ降低,但关于IGF-Ⅱ的水平则报道甚少。本文采用特异性酶联免疫分析法,测定了慢性肝病包括原发性肝细胞性肝癌患者(PHC)血清中IGF-Ⅱ的水平,以探讨其诊断意义。受试者包括57名健康志愿者,PHC 病人17例,肝硬化50例,慢性肝炎29例,以及非肝脏性疾病108例.所有受试者进行下列检查:1.血生化测定;2.血清IGF-Ⅱ的酶联免疫分析测定;3.血清IGFⅠ放免测定;4.血清GH、T_3和T_4测定;5.其他实验室检查,包括血浆蛋白、白蛋白、总胆红素、胆碱酯酶、GOT、GPT、ALP、总胆酸、凝血试验和靛青绿(ICG)滞留试验。结果:健康对照组血清IGF-Ⅱ的平均值为830±4png/ml;慢性肝炎(538±51),肝硬化(427±45)和PHC(260±41)较对照组明显降低;非肝脏性疾病组则
Insulin-like growth factor Ⅱ (IGF-Ⅱ) is a polypeptide of 67 amino acids, mainly synthesized and secreted by the liver. Some studies have shown that liver disease serum IGF-Ⅰ decreased, but on the level of IGF-Ⅱ report little. In this paper, the specific enzyme-linked immunosorbent assay was used to determine the level of IGF-II in the serum of patients with chronic liver disease, including primary hepatocellular carcinoma (PHC), so as to explore its diagnostic significance. Subjects included 57 healthy volunteers, 17 PHC patients, 50 cirrhosis, 29 chronic hepatitis, and 108 non-hepatic diseases. All subjects underwent the following examinations: 1. Blood biochemical assays; 2. Serum IGF-Ⅱ enzyme-linked immunosorbent assay; 3. Serum IGFⅠ radioimmunoassay; 4. Serum GH, T_3 and T_4 assays; 5. Other laboratory tests, including plasma proteins, albumin, total bilirubin, , GOT, GPT, ALP, total bile acid, clotting test and indigo green (ICG) retention test. Results: The mean value of serum IGF-Ⅱ in healthy control group was 830 ± 4png / ml; the levels of chronic hepatitis (538 ± 51), cirrhosis (427 ± 45) and PHC (260 ± 41) were significantly lower than those in control group; Disease group then