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目的 :观察去神经损伤后海马中胰岛素样生长因子2(insulin-like growth factor 2,IGF-2),碱性成纤维细胞生长因子2(basic fibroblastic growth factor 2,FGF-2),神经生长因子(nerve growth factor,NGF)和睫状神经营养因子(ciliary neurotrophic factor,CNTF)等神经营养因子在急性期内各时间点的基因表达变化。方法 :将28只SD大鼠随机分成双侧穹窿海马伞切割后0.5、1、2、6、12及24 h组和假手术组,在穹窿海马伞切割上述各时间点后,分别取各组大鼠海马组织,并提取总RNA,利用半定量逆转录-聚合酶链反应(reverse transcriptase polymerase chain reaction,RT-PCR)法分析切割穹窿海马伞后海马内IGF-2、FGF-2、NGF、CNTF的m RNA表达变化情况。结果:在切割大鼠穹窿海马伞后急性期的早期阶段,0.5 h组CNTF m RNA的表达量与假手术组相比明显增加(P<0.05);NGF m RNA的表达水平在12 h和24 h时与假手术组相比明显下降(P<0.05);IGF-2 m RNA的表达水平在24 h时与假手术组相比显著减少(P<0.05);FGF-2m RNA从海马伞切割后1、2、6、12和24 h均较假手术组下降(P<0.05)。结论 :切割穹窿海马伞后CNTF m RNA急性期极早期表达较假手术组上调,可能与急性期海马对应激性损伤的保护作用有关,这将为更深层次地研究海马在去神经支配损伤后神经再生与神经修复提供新的思路。
OBJECTIVE: To observe the effects of dexamethasone on the expression of insulin-like growth factor 2 (IGF-2), basic fibroblast growth factor 2 (FGF-2), nerve growth factor (NGF) and ciliary neurotrophic factor (CNTF) and other neurotrophic factors at different time points during the acute phase. Methods: Twenty-eight Sprague-Dawley rats were randomly divided into three groups: 0.5, 1, 2, 6, 12 and 24 h after the fimbria fornix transection, and sham operation group. After the above- Rat hippocampus, total RNA was extracted and the expression of IGF-2, FGF-2 and NGF in the hippocampus were detected by reverse transcriptase polymerase chain reaction (RT-PCR) CNTF m RNA expression changes. Results: The expression of CNTF m RNA in 0.5 h group was significantly increased compared with that in sham operation group (P <0.05) at the early stage of acute phase after excising rat fimbria fornix. The expression level of NGF m RNA at 12 h and 24 (P <0.05). The expression of IGF-2 mRNA decreased significantly at 24 h compared with that in sham operation group (P <0.05). FGF-2 mRNA was cut from the hippocampus 1, 2, 6, 12 and 24 h after the operation were lower than the sham group (P <0.05). CONCLUSION: The early and late expression of CNTF m RNA in the acute phase after Fimbria fornix transection is higher than that in the sham-operated group, which may be related to the protective effect of hippocampus on acute stress injury in hippocampus. Regeneration and nerve repair provide new ideas.