取材母株的栽培环境、取材季节和部位是影响金叶风箱果初代离体培养外植体褐化的主要因素。取材母株放入温室和在相对弱光照下生长 ,其外植体的褐化率比在露地全光照下栽培母株低 ;3月中旬取枝条下部冬芽和 5月上中旬取栽培在温室的母株上部腋生枝条嫩枝段作外植体的褐化率低 The cultivation environment, the season and the location of the parental plants were the main factors affecting the browning of explants in vitro of the first generation of gold leaf bellows. The parental plants were placed in the greenhouse and grown under relatively low light. The browning rate of explants was lower than that of the cultivated parent plants under the field exposure. In mid-March, the lower shoots of winter branches were harvested and in mid- The browning rate of shoots of axillary branches in the upper part of the plant as explants is low