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目的：实验观察不同浓度乙酰胆碱（ＡＣｈ，１０－１０～１０－５ｍｏｌ／Ｌ）对大鼠Ｂ细胞功能的作用，并初步探讨其作用机制。方法：在离体条件下，利用细胞培养方法，以绵羊红细胞（ＳＲＢＣ）刺激大鼠肠系膜淋巴结Ｂ细胞转化成抗体形成细胞（ＡＦＣ），然后检测其抗体生成量，并以此作为衡量Ｂ细胞功能的指标。结果：（１）１０－１０～１０－７ｍｏｌ／ＬＡＣｈ能显著抑制Ｂ细胞功能，其中１０－８和１０－７ｍｏｌ／ＬＡＣｈ的作用较强，而１０－６和１０－５ｍｏｌ／ＬＡＣｈ无明显的抑制作用。（２）Ｍ型胆碱能受体拮抗剂阿托品（１０－７和１０－６ｍｏｌ／Ｌ）可完全阻断ＡＣｈ抑制Ｂ细胞功能的作用。结论：ＡＣｈ可非浓度依赖性地抑制大鼠的Ｂ细胞功能，此作用可能由Ｂ细胞上的Ｍ型胆碱能受体介导。
Objective: To observe the effect of different concentrations of acetylcholine (ACh, 10-10 ~ 10-5 mol / L) on the function of B cells in rats, and to explore its mechanism. Methods: In vitro, BMSCs were stimulated by sheep erythrocytes (SRBC) to form antibody-producing cells (AFCs), then the antibody production was measured and used as a measure of B cell function index of. Results: (1) 10-10 ~ 10-7mol / LACh could significantly inhibit the function of B cells, of which 10-8 and 10-7mol / LACh had stronger effect, while 10-6 and 10-5mol / LACh had no obvious inhibition effect. (2) Atropine (10-7 and 10-6mol / L), a type M cholinergic receptor antagonist, completely blocked the effect of ACh on B cell function. Conclusion: ACh can inhibit B-cell function in a concentration-dependent manner in rats, which may be mediated by M-type cholinergic receptors on B cells.