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收集马来西亚产的水半夏(Typhonium flagelliforme)全草,粉碎后以甲醇(3×5L)室温提取,提取液经干燥后,混悬于水中,以乙酸乙酯提取3次,再以硅胶柱纯化分离,并用逐渐增加甲醇浓度的氯仿溶液洗脱,每5ml洗脱液作为一个组分,收集Rf为0.15和0.72的组分,经反相HPLC纯化,用乙腈-
The whole plant of Typhonium flagelliforme from Malaysia was collected, crushed and extracted with methanol (3×5L) at room temperature. The extract was dried, suspended in water, extracted three times with ethyl acetate, and purified by silica gel column. Separate and elute with a chloroform solution that gradually increases the concentration of methanol. Collect the components with an Rf of 0.15 and 0.72 for each 5 ml of the eluate. Purify by reverse phase HPLC using acetonitrile-