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应用人白细胞抗原（ＨＬＡ）基因的聚合酶链反应－限制性片段长度多态性核苷酸分型技术，以等位基因特异性的限制性内切酶（ＡｐａｌⅠ、 ＢｓａｊⅠ、 ＨｐｈⅠ、 ＦｏｋⅠ、 Ｍｂｏ Ⅱ、 ＭｕｌⅠ）消化 ＤＱＡ１座位特异的 ＰＣＲ扩增产物，对５０例胃溃疡患者、 ５０例正常人的ＨＬＡ－ＤＱＡ１等位基因进行分析，旨在研究武汉地区汉族人ＨＬＡ－ＤＱＡ１基因与胃溃疡的相互关系。发现胃溃疡患者ＤＱＡ１＊０３０１显著增加（Ｐ＜０．０５），而ＤＱＡ１＊０１０２显著下降（Ｐ＜０．０５）。提示＊ＱＡ１＊０３０１和＊ＱＡ１＊０１０２与胃溃疡有一定关联，胃溃疡患者与正常人之间存在着免疫遗传异质性的养异。
The allele-specific restriction endonucleases (ApalⅠ, BsajⅠ, HphⅠ, FokⅠ, Mbo) were cloned by polymerase chain reaction-restriction fragment length polymorphism (PCR) using human leukocyte antigen (HLA) Ⅱ, Mul Ⅰ) to digest DQA1 locus-specific PCR amplification products. HLA-DQA1 alleles in 50 patients with gastric ulcer and 50 normal individuals were analyzed to investigate the relationship between HLA-DQA1 gene and gastric ulcer Interrelationship. DQA1 * 0301 was found to be significantly increased in patients with gastric ulcer (P <0.05), while DQA1 * 0102 was significantly decreased (P <0.05). Prompt * QA1 * 0301 and * QA1 * 0102 and gastric ulcer have a certain correlation, gastric ulcer patients and normal there is a heterogeneous immune genetic heterogeneity.