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采集闽江口鳝鱼滩优势植物芦苇(Phragmites australis)、短叶茳芏(Cyperus malaccensis Lam.var.brevifolius Bocklr)和互花米草(Spartina alterniflora)沼泽的土壤样品,添加土壤醋酸发酵途径产甲烷的特殊抑制剂——磷酸盐缓冲液以及不同甲烷产生基质(醋酸、H2/CO2、甲醇、甲胺和三甲胺),在原位温度下室内厌氧培养5d。结果表明,芦苇、短叶茳芏和互花米草沼泽醋酸发酵途径甲烷产生量分别为0.0032～0.0105μg/(g?d),0.0018～0.0093μg/(g?d)和0.0021～0.0147μg/(g?d),生长有3种植物的沼泽土壤0～50cm土层醋酸发酵途径产甲烷量分别占其各自甲烷产生总量的53.7%,53.4%和65.2%,其中,0～20cm土层是沼泽土壤甲烷产生的主要土层,分别占0～50cm土层甲烷产生总量的51.0%,48.7%和56.6%,甲烷产生量随着土壤深度的增加而减小。添加醋酸和3种甲基类化合物均不同程度上提高了沼泽土壤甲烷的产生量,其中甲醇的刺激作用最明显,添加H2/CO2的处理影响很小。
The soil samples from Phragmites australis, Cyperus malaccensis Lam. Var. Brevifolius Bocklr and Spartina alterniflora swamps were collected, and the soil acetic acid fermentation pathway was added to produce methane The special inhibitor - phosphate buffer and different methane production substrates (acetic acid, H2 / CO2, methanol, methylamine and trimethylamine) were incubated anaerobically for 5 days in situ. The results showed that the yields of methane in the fermentations of Phragmites australis, M. scrofa and S. alterniflora were 0.0032-0.0105 μg / (g? D), 0.0018-0.0093 μg / (g? D) and 0.0021-0.0147 μg / (g · d). The methane production of 0 ~ 50cm layer of swamp soil grown in three kinds of plants accounted for 53.7%, 53.4% and 65.2% of the total amount of methane production respectively. Among them, 0 ~ 20cm soil layer Is the main soil methane produced in marsh soil, accounting for 51.0%, 48.7% and 56.6% of the total methane production in 0 ~ 50cm soil layer respectively. The methane production decreases with the increase of soil depth. The addition of acetic acid and three kinds of methyl compounds increased the production of methane in swamp soil to some extent. Among them, the stimulation effect of methanol was the most obvious, and the effect of adding H2 / CO2 was little.