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研究建立了用超高效液相色谱-二极管阵列检测器测定16中EPA优控多环芳烃(PAHs)的方法,考察了BEH C18 column(100×2.1 mm i.d.,1.7μm),BEH SHIELD RP18(50×2.1 mm i.d.,1.7μm),BEH C18(50×2.1 mm i.d.,1.7μm),BEH C8(50×2.1 mm i.d.,1.7μm)和BEH Phenyl(50×2.1 mm i.d.,1.7μm)等5种色谱柱分别用乙腈-水或甲醇-水分离PAHs的效果,综合考察分离效果和时间,选择BEH C18 column(100×2.1 mm i.d.,1.7μm)色谱柱用乙腈-水分离PAHs,16种PAHs在8.1 min内流出,在12 h内和1 m内,PAHs的保留时间标准偏差分别低于0.01、0.04 min,检出限在5~40 pg之间,定量检测限10~200 pg之间,线性相关系数(r2)在0.9993~0.9997之间。
A method was developed for the determination of PAHs in 16 high-performance liquid chromatography-diode array detectors. The BEH C18 column (100 × 2.1 mm id, 1.7 μm) and BEH SHIELD RP18 5 × 2.1 mm id, 1.7 μm), BEH C18 (50 × 2.1 mm id, 1.7 μm), BEH C8 (50 × 2.1 mm id, 1.7 μm) and BEH Phenyl The effect of PAHs on the column was separated by acetonitrile-water or methanol-water respectively. The separation effect and time were comprehensively investigated. PAHs were separated by acetonitrile-water on a BEH C18 column (100 × 2.1 mm id, 1.7 μm) The standard deviation of retention time of PAHs was less than 0.01 and 0.04 min in 12 h and 1 m, respectively. The limits of detection were between 5 and 40 pg, and the limits of quantification were between 10 and 200 pg. The linearity The correlation coefficient (r2) is between 0.9993 and 0.9997.