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目的对株洲市2005-2009年流行性感冒的病原学检测结果进行分析。方法2005-2009年采集哨点医院流感样病例(ILI)的咽拭子标本,采用狗肾传代细胞(MDCK)进行流感病毒分离,采用血凝抑制实验(HI)和实时荧光(RT-PCR)进行病毒型别鉴定。结果五年共检测标本3840份,分离到各型流感病毒271株,其中72株因血凝滴度<1∶8未能鉴定,鉴定的199株结果为A(H3N2)72株,占36.18%;A(H1N1)75株,占37.68%;B型52株,占26.13%。结论近年来,株洲市市区流感监测哨点的流感样病例病毒分离阳性率为7.06%。2005年10月-2006年以A(H1N1)亚型为流行毒株、2007年以A(H3N2)亚型为主要流行毒株,2008-2009年10月31日止A(H1N1)亚型、A(H3N2)亚型、B型并存。
Objective To analyze the epidemiological results of influenza in Zhuzhou City from 2005 to 2009. Methods Throat swab samples of influenza-like illness (ILI) from sentinel hospital were collected during 2005-2009. Influenza virus was isolated from dog’s passaged kidney cells (MDCK) by hemagglutination inhibition (HI) and real-time fluorescence (RT- Virus type identification. Results A total of 3840 specimens were obtained in 5 years, of which 271 strains were isolated. Among them, 72 strains were identified as hemagglutination titer <1: 8, and 72 strains were identified as A (H3N2), accounting for 36.18% 75 strains of A (H1N1) accounted for 37.68%, 52 strains of B type accounted for 26.13%. Conclusions In recent years, the positive rate of influenza virus-like cases in urban influenza surveillance sentinel sites in Zhuzhou City was 7.06%. From October 2005 to 2006, the A (H1N1) subtype was taken as the epidemic strain. The A (H3N2) subtype was the main epidemic strain in 2007. The A (H1N1) subtype was on October 31, 2008, A (H3N2) subtype, B co-exist.