论文部分内容阅读
目的 建立大鼠胰腺腺泡细胞癌癌前病变的动物模型,了解雌激素对该癌前病变及K-ras基因突变的影响.方法 雄性Wistar大鼠单次腹腔注射重氮乙酰丝氨酸30mg/kg,部分大鼠2个月后开始肌肉汪射戊酸雌二醇0.18mg/100g×2周,4个月后,常规取胰腺组织行光镜和电镜检查,并应用PCR-SSCP法检测K-ras基因突变情况.结果 单纯给予重氮乙酰丝氨酸的大鼠胰腺腺泡细胞癌癌前病变发生率100%,K-ras基因突变率42.1%;经雌激素处理的大鼠胰腺腺泡细胞癌癌前病变发生率92.4%,K-ras基因突变率25.6%.结论 重氮乙酰丝氨酸可成功诱导大鼠胰腺腺泡细胞癌癌前病变,K-ras基因突变在此过程中属早期事件.雌激素一定程度上可抑制该癌癌前病变的发生发展.
Objective To establish an animal model of precancerous lesions of rat pancreatic acinar cell carcinoma to understand the effects of estrogen on this precancerous lesion and K-ras gene mutation. Methods Male Wistar rats were given a single intraperitoneal injection of diazoacetylserine 30 mg/kg. Two months later, rats began to develop estradiol valerate 0.18 mg/100 g for 2 weeks. Four months later, routine pancreatic tissue examination was performed with light and electron microscopy, and PCR-SSCP was used to detect K-ras. Gene mutations.Results Rats with diazoacetylserine alone had 100% precancerous lesions of pancreatic acinar cell carcinoma, and 42.1% of K-ras mutations. Estrogen-treated rats had precancerous pancreatic acinar cell carcinoma. The incidence rate of lesions was 92.4%, and the mutation rate of K-ras gene was 25.6%. Conclusion Diazacetylserine can successfully induce precancerous lesion of rat pancreatic acinar cell carcinoma. K-ras gene mutation is an early event in this process. Estrogen must be To a certain extent, it can inhibit the occurrence and development of this precancerous lesion.