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目的评估骨吸收抑制剂对猕猴皮质骨骨形态计量学指标的影响。方法 20只雌性猕猴(18~22岁)按体重随机分为5组:对照组(CNT)、低剂量依降钙素组[0.5 U/(kg·d),ELL]、高剂量依降钙素组[5 U/(kg·d),ELH]、低剂量阿仑膦酸钠盐组[10μg/(kg·d),ALL]和高剂量阿仑膦酸钠盐组[100μg/(kg·d),ALH]。皮下注射给药29周,每周2次。处死前静脉注射钙黄绿素,处死后取左侧股骨中段横截面进行骨形态计量学检测。结果 5组左侧股骨截面总面积(Tt.Ar)、皮质骨面积(Ct.Ar)、髓腔面积(Ma.Ar)、类骨质周长(O.Pm)和类骨质宽度(O.Wi)的差异均无统计学意义(均P>0.05)。用药各组皮质骨骨吸收腔周长(E.Pm)均较对照组明显降低(均P<0.05)。ALH组骨矿沉积率(MAR.)低于对照组(P=0.049)。ELL、ALL、ALH组修正后的沉积速率(AJ.AR)低于对照组(P=0.040、0.018、0.09)。各组骨矿化延迟时间(Mlt)、修正后的骨矿化延迟时间(O.Wi/AJ.AR.)和骨形成率(BFR)间差异无统计学意义(P=0.171、0.110、0.122)。结论降钙素及阿仑膦酸钠通过抑制皮质骨破骨细胞功能抑制皮质骨的骨重建,二膦酸盐作用强于降钙素。降钙素及阿仑膦酸钠能不同程度地影响MAR,但对Mlt及总BFR影响不大。
Objective To evaluate the effect of bone resorption inhibitor on cortical mesenchymal bone morphometrics in macaque. Methods Twenty females (18-22 years old) were randomly divided into five groups according to body weight: control group (CNT), low dose calcitonin group [0.5 U / (kg · d), ELL] ELU], low dose alendronate sodium salt group [10μg / (kg · d), ALL] and high dose alendronate sodium salt group [100μg / (kg · D), ALH]. Subcutaneous injection for 29 weeks, 2 times a week. Before the death of intravenous calcein injection, after sacrifice and left cross section of the middle of the femur bone morphology measurement. Results The left femur total area (Tt.Ar), cortical area (Ct.Ar), medullary cavity area (Ma.Ar), peroidoid bone length (O.Pm) and osteoid width .Wi) had no statistical significance (all P> 0.05). The cortical bone resorption lumen perimeter (E.Pm) in each group was significantly lower than that in the control group (all P <0.05). ALS group bone mineral deposition rate (MAR) was lower than the control group (P = 0.049). The corrected deposition rate (AJ.AR) in ELL, ALL, and ALH groups was lower than that in the control group (P = 0.040, 0.018, 0.09). There were no significant differences in the bone mineralization delay time (Mlt), modified bone mineralization delay time (O.Wi / AJ.AR.) And bone formation rate (BFR) between the two groups (P = 0.171,0.110,0.122 ). Conclusion Calcitonin and alendronate inhibit the cortical bone remodeling by inhibiting cortical osteoclast function, and the effect of bisphosphonates is stronger than that of calcitonin. Calcitonin and alendronate could affect MAR to varying degrees, but had little effect on Mlt and total BFR.