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[目的]探讨人单核细胞对煤焦沥青烟提取物(CTPE)处理人永生化支气管上皮细胞中c-Jun m RNA表达的影响。[方法]用3μg/m L CTPE刺激人永生化支气管上皮细胞(CTPE组),及人单核细胞共培养的人永生化支气管上皮细胞(CC组),设立二甲基亚砜对照组;分别收集各组1、5、10、15、20代细胞。将CC组第9代细胞分别常规培养至10代(CC10组)、15代(CC15组),加入100μg/m L肿瘤坏死因子-α中和抗体培养至15代(中和抗体组)。应用实时荧光定量聚合酶链式反应检测各组细胞中c-Jun m RNA的表达水平。[结果]15、20代CC组人永生化支气管上皮细胞c-Jun m RNA相对表达水平高于CTPE组和二甲基亚砜对照组(均P<0.05);中和抗体组c-Jun m RNA的相对表达水平高于CC10组,低于CC15组(均P<0.05)。[结论]人单核细胞可能通过肿瘤坏死因子-α调控CTPE诱导人永生化支气管上皮细胞c-Jun m RNA的表达。
[Objective] To investigate the effect of human mononuclear cells on c-Jun m RNA expression in human immortalized bronchial epithelial cells treated with coal tar pitch smoke extract (CTPE). [Methods] Human immortalized bronchial epithelial cells (CTPE group) and human immortalized bronchial epithelial cells (CC group) co-cultured with human monocytes were stimulated with 3 μg / mL CTPE and DMSO control groups were established respectively; Groups of 1, 5, 10, 15 and 20 generations of cells were collected. The 9th generation cells of CC group were routinely cultured to the 10th generation (CC10 group) and the 15th generation (CC15 group) respectively, and then cultured for 15 generations (neutralizing antibody group) by adding 100μg / ml tumor necrosis factor-α neutralizing antibody. The expression of c-Jun m RNA in each group of cells was detected by real-time fluorescence quantitative polymerase chain reaction. [Results] The relative expression level of c-Jun m RNA in immortalized bronchial epithelial cells of 15,20 generation CC group was higher than that in CTPE group and dimethyl sulfoxide control group (all P <0.05). The neutralization antibody group c-Jun m The relative expression level of RNA was higher in CC10 group than in CC15 group (all P <0.05). [Conclusion] Human mononuclear cells may regulate the expression of c-Jun m RNA induced by CTPE in human immortalized bronchial epithelial cells by TNF-α.