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目的观察血小板衍生生长因子(PDGF)等生长因子对鼠成骨细胞内Ca2+脏度变化的影响。方法酶消化法体外分离培养胎儿鼠成骨细胞,应用ACAS检测成骨细胞内Ca2+脓度的变化。结果PDGF可促进成骨细胞内Ca2+脓度的瞬时增加;PDGF与BMP、TGF-β联合应用对成骨细胞Ca2+浓度升高有协同作用;ACAS能准确测量胞内Ca2+浓度的动态变化。结论PDGF可促进成骨细胞内Ca2+浓度的增加.且与BMP、TGF-β有协同效应。
Objective To observe the effects of growth factors such as platelet derived growth factor (PDGF) on the changes of intracellular Ca2 + in rat osteoblasts. Methods Cultured fetal rat osteoblasts were isolated by enzymatic digestion method, and the changes of Ca2 + purulence in osteoblasts were detected by ACAS. Results PDGF could promote the transient increase of Ca2 + purulence in osteoblasts. The combination of PDGF and BMP and TGF-β had a synergistic effect on the increase of Ca2 + concentration in osteoblasts. ACAS could accurately measure the intracellular Ca2 + concentration. Conclusion PDGF can increase the concentration of Ca2 + in osteoblasts. And with BMP, TGF-β have a synergistic effect.