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目的观察缺氧模拟物去铁胺(DFO)对人源肠道Caco-2细胞成纤维细胞生长因子21(FGF21)表达的影响。方法分别用50、100、150、200μmol/L的DFO处理Caco-2细胞12 h,同时用200μmol/L的DFO处理Caco-2细胞4、8、12 h,RT-PCR法检测细胞中的FGF21 mRNA。分别用缺氧诱导因子(HIF)抑制剂和ROS抑制剂预处理Caco-2细胞,再用DFO继续处理,RT-PCR法检测细胞中的FGF-21 mRNA。结果随DFO浓度增加和作用时间延长,Caco-2细胞FGF21 mRNA相对表达量逐渐降低(P均<0.05)。HIF抑制剂预处理后,Caco-2细胞FGF21相对表达量依然降低(P均<0.05);ROS抑制剂预处理后,Caco-2细胞FGF21 mRNA相对表达量未出现明显降低(P均>0.05)。结论 DFO对肠道Caco-2细胞FGF21的表达有抑制作用,该作用与缺氧产生HIF无关,而与ROS有关。
Objective To observe the effect of hypoxia mimetic deferoxamine (DFO) on the expression of fibroblast growth factor 21 (FGF21) in human intestinal Caco-2 cells. Methods Caco-2 cells were treated with 50, 100, 150 and 200 μmol / L DFO for 12 h, while Caco-2 cells were treated with 200 μmol / L DFO for 4, 8 and 12 h, respectively. mRNA. Caco-2 cells were pretreated with hypoxia-inducible factor (HIF) inhibitors and ROS inhibitors, and then treated with DFO. The FGF-21 mRNA was detected by RT-PCR. Results The relative expression of FGF21 mRNA in Caco-2 cells gradually decreased with the increase of DFO concentration and the prolonged action time (all P <0.05). After pretreatment with HIF inhibitor, the relative expression of FGF21 in Caco-2 cells was still decreased (all P <0.05). The relative expression of FGF21 mRNA in Caco-2 cells was not significantly decreased after pretreatment with ROS inhibitor (all P> 0.05) . Conclusion DFO can inhibit the expression of FGF21 in intestinal Caco-2 cells. The effect of DFO is not related to hypoxia-producing HIF but not to ROS.