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目的 探索用准分子激光切削技术制备视网膜单层视细胞植片，经内入路视网膜下腔的单层视细胞成片移植。方法用准分子激光对大鼠视网膜进行切削，制取单层视细胞植片，此后，按内入路手术方法进行了兔视网膜下腔的异种移植。结果 切削后所得视细胞植片由单层视细胞组成，结构完整，包括外丛状层、外核层和外节层；视细胞植片经明胶包埋后被准确植入宿主视网膜下腔中，移植术后第１，２天宿主视网膜未能复位，呈脱离状态，移植物没能与视网膜色素上皮层相贴；移植后１０天，宿主视网膜复位，视细胞移植片平铺于宿主视网膜下腔中，植片视细胞外节与宿主视网膜色素上皮层相贴，未见明显免疫排异现象。结论 准分子激光制备单层视细胞植片方法简单、可行；初步观察到内入路单层视细胞成片移植后，视细胞植片能够在宿主视网膜下腔中以正常生理位置存活；视网膜下腔为理想的视网膜移植的受位。
Objective To explore the use of excimer laser cutting technique for the preparation of retinal monolayer visual cell grafts and transplanting the monolayer visual cells into the subretinal space through the approach. Methods The rat retina was excised by excimer laser to make monolayer retinal cells. After that, the rabbit subretinal xenotransplantation was performed by intra-operative approach. Results After cutting, the visual cell grafts were composed of monolayer visual cells with complete structure, including extra-plexiform layer, extranuclear layer and extra-node layer. The visual cell grafts were accurately implanted into the subretinal space of the host after gelatin-embedded , The first and second days after transplantation, the host retina failed to reset, was detachment, the graft failed to patch with the RPE; host 10 days after transplantation, the retina reset, depending on the cell graft in the host subretinal space In the graft, the extracellular stoma was attached to the host retinal pigment epithelium and no obvious immune rejection occurred. Conclusions The method of excimer laser to prepare monolayer retinal cells is simple and feasible. It is initially observed that after implantation of mononuclear retinal cells, the optic disc can survive in the normal subretinal space in normal subretinal space. Cavity for the ideal retinal transplant site.