香蕉枯萎病是由尖孢镰刀菌(Fusariumoxysporum)引起的一种维管束坏死的系统性病害。田间病株自苗期感染到开始发病要5—6个月,而人工接种大苗也要2—9个月才表现症状(Stover,1959)。我们曾用块茎小苗接种,潜伏期比按种大苗短,表明接种小的蕉苗可以缩短病害的潜伏期,据此进一步进行了组培苗伤根接种试验,以寻找一种准确、快速、简便的接种方法。(一)材料与方法龙牙蕉组织培养苗通过不定芽增殖的方法培育。假植于花盆40—45天,苗高12—18厘米。病原菌从中山龙牙蕉病株中分离并经致病 Banana wilt disease is a systemic disease of vascular bundle necrosis caused by Fusarium oxysporum. Infected plants in the field have a disease onset of 5-6 months from the start of the seedling stage, and 2-9 months after the artificial inoculation of the seedlings. (Stover, 1959). We used to vaccinate tuberous seedlings with a shorter incubation period than that of seedlings, indicating that inoculation of small banana seedlings can shorten the incubation period of the disease. Based on this, we further conducted root inoculation experiments with tissue culture seedlings to find an accurate, rapid and easy inoculation method. (A) Materials and Methods Tissue Culture Adenophora tissue culture by adventitious bud propagation method. Fake plants planted in pots 40-45 days, 12-18 cm tall. The pathogen was isolated from Zhongshan dragon-tooth plant and was pathogenic