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应用SSR分子标记技术,研究了以不同抗性转基因741杨为食物的杨扇舟蛾实验种群的遗传分化,探讨以转Bt基因杨树作为食物对靶标昆虫的胁迫效应.利用筛选出的10对引物,分别对经取食转基因741杨高抗品系‘Pb29’、中抗品系‘Pb17’及未转基因杨(对照)筛选出的杨扇舟蛾实验种群进行遗传多样性和遗传分化研究.结果表明:10对引物共检测到76条等位基因,平均等位基因7.6个,平均有效等位基因为2.2,平均观测杂合度为0.5167,平均期望杂合度为0.5167,平均多态位点百分率达96.7%.经取食转基因741杨筛选出的杨扇舟蛾实验种群的遗传多样性水平显著高于未转基因种群,且取食转基因741杨的杨扇舟蛾与CK样本间的遗传相似性最低,表明经取食转Bt基因杨树的杨扇舟蛾实验种群遗传多样性有增高趋势.基于SSR分析,转基因741杨对杨扇舟蛾实验种群的胁迫筛选效应明显.
The SSR molecular marker technique was used to study the genetic differentiation of the experimental population of the genus Brachion to investigate the stress effect on the target insects using the transgenic Bt poplar as food. Ten pairs of primers , Respectively, to study the genetic diversity and genetic differentiation of the experimental population of the genus Brachionacea which were selected by using the high resistance strain ’Pb29’, the medium resistant strain ’Pb17’ and the non-transgenic Yang (control) of the transgenic poplar 741.The results showed that 10 pairs of primers A total of 76 alleles were detected, with an average of 7.6 alleles, the average effective allele was 2.2, the average observed heterozygosity was 0.5167, the average expected heterozygosity was 0.5167, the average percentage of polymorphic loci was 96.7% The results showed that the genetic diversity of the experimental population of B.angua was significantly higher than that of the non-transgenic population, and the genetic similarity between C. salina and CK samples fed the transgenic poplar 741 Yang was the lowest, indicating that the transgenic Bt Genetic diversity of the Populus brasiliensis population tested showed an increasing trend.According to SSR analysis, the effects of transgenic Poplar 741 on stress screening of the experimental population of the genus Brachionia were significant.