论文部分内容阅读
目的：探讨利拉鲁肽对大鼠脊髓损伤（SCI）神经的修复作用及机制。方法将雌性大鼠分为利拉鲁肽干预组、正常对照组，每组24只。采用大鼠脊髓动脉瘤夹夹伤法建立大鼠脊髓损伤模型。术后分别给予利拉鲁肽（0．8 mg ／kg，0．5 mL）、生理盐水（0．5 mL）。术后2 d 应用细胞凋亡检测技术检测神经细胞损伤程度，术后3 d 采用 Western blotting 蛋白印迹技术检测损伤脊髓组织中白细胞介素-1β（IL-1β）、白细胞介素-6（IL-6）表达水平，术后14 d 对损伤脊髓组织行 HE 染色观察组织形态变化并计算损伤空洞面积，术后3 d 至28 d 定期记录大鼠后肢行为学 BBB 评分。结果SCI 后利拉鲁肽干预组细胞凋亡、炎性因子表达、空洞面积计算均较正常对照组降低（P ＜0．01），利拉鲁肽干预组双后肢 BBB 评分同期较正常对照组高（P ＜0．01）。结论脊髓损伤后给予利拉鲁肽会降低细胞凋亡、急性炎症反应，有利于后期神经组织修复以及运动动能恢复。“,”Objective To investigate the role and mechanism of liraglutide in the repair of spinal cord injury (SCI)in rats.Methods A total of 48 rat models of clip compressive SCI were established and then divided into 2 groups:lira-glutide treatment group (n =24)and normal control group (n =24),which were injected with liraglutide (0.8 mg /kg, 0.5 mL)and physiological saline (0.5 mL)respectively after the operation.The apoptosis of neurocytes was detected with TUNEL method on day 2 after operation,the expressions of interleukin-1βand interleukin-6 were detected with Western blotting protein imprinting technology on day 3,the structure of injured spinal cord tissue was observed with HE staining on day 14,and the BBB score of hind limbs of rats behavior were recorded from day 3 to day 28.Results The rate of cell apoptosis,expression of inflammatory factors,and cavity area of the liraglutide treatment group were lower than those of the control group (all P <0.01),while the BBB scores of both hind limbs were higher than those in the control group at the same period (P <0.01).Conclusion Liraglutide treatment after spinal cord injury may reduce cell apoptosis acute inflammation,and is conducive to the late repair of nerve cells and the recovery of motor function.