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目的观察清脂颗粒对蛋黄乳高脂血模型小鼠药诱实验。方法雌雄各半的昆明种小鼠60只,随机分为空白对照组、阳性药对照组、模型对照组、清脂颗粒3个受试剂量组[18.4g/(kg·d)]、[9.2g/(kg·d)]、[4.6g/(kg·d)],每组各10只,给药7d后,除空白对照组,其他各组分别腹腔注射80%蛋黄乳液0.1mL/10g,禁食10h后,采用常压耐缺氧实验方法,观察小鼠缺氧存活时间,测定血清中丙二醛(MDA)、一氧化氮(NO)的含量。结果用药后小鼠缺氧存活时间延长,蛋黄乳高脂模型小鼠血清中MDA、NO有明显升高。给药组血清中MDA、NO中明显低于蛋黄乳高脂模型组,并具有统计学意义(P<0.01)。结论清脂颗粒具有明显提高蛋黄乳高脂血模型小鼠耐缺氧能力的作用。
Objective To observe the drug-induced experiment of Qingzhi Granules on mice with hyperlipemia of egg yolk. Methods Sixty male and sixty Kunming mice were randomly divided into blank control group, positive drug control group, model control group, Qingzhi Granule 3 test dose groups [18.4g/(kg·d)], [9.2 g/(kg·d)] and [4.6g/(kg·d)], 10 in each group. After 7 days of administration, except for the blank control group, other groups were injected intraperitoneally with 80% egg yolk emulsion 0.1 mL/10 g. After fasting for 10 hours, the hypoxia-tolerance experimental method was used to observe the hypoxic survival time of mice, and the content of malondialdehyde (MDA) and nitric oxide (NO) in serum was measured. Results The duration of hypoxic survival in mice was prolonged after administration, and the serum levels of MDA and NO in mice with high cholesterol in egg yolk were significantly higher. The serum levels of MDA and NO in the drug-administered group were significantly lower than those in the egg-yolk-milk model group (P<0.01). Conclusion Qingzhi Granules can significantly increase the hypoxic tolerance of mice with hyperlipemia in egg yolk.