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四川省南充县良种场1983年承担中华猕猴桃人工组织培养的研究任务,先后接种868支试管,从茎、叶外植体诱导出愈伤组织140块,分化出芽丛88丛,培养出试管整体绿色植株560株,至今年四月中旬,通过炼苗,室内再植,逐级转入大田288株,已成活151株。该良种场在研究过程中,有严格消毒灭菌、诱导愈伤组织、分化出芽、继代培养、培育试管整体株等项工作。材料选择一年生上部枝条和初生出不久的小嫩叶片,经清洗灭菌处理后,切成0.5～1cm~2的小块,在严
In 1983, Nanchong County, Sichuan Province, on the species field to undertake artificial tissue culture kiwifruit research mission, inoculation of 868 test tubes from the stem and leaf explants to induce callus 140, differentiation of bud plexus 88 plexus, cultivate the overall green tube plant 560 By mid-April this year, 288 plants were transferred to Daejeon through refining and indoor replantation, and 151 plants have survived. During the course of the research, there are strict work on disinfection and sterilization, callus induction, bud differentiation, subculture and cultivation of whole tube of test tube. Material selection of the upper branches and the first birth of young leaves soon after the small, after cleaning and sterilization, cut into small pieces of 0.5 ~ 1cm ~ 2, strictly