论文部分内容阅读
为评价陕甘山桃(Prunus davidiana var.potaninii Rehd)对根癌病的抗性,以实生钵苗为试材,采用人工接种的方法研究了其对根癌土壤杆菌(Agrobacterium tumefaciens Conn)和发根土壤杆菌(A.rhizogenes Conn)的抗性。结果表明:接种后60d,接种根癌土壤杆菌的陕甘山桃的株最大瘤径范围为0～10.1mm,平均瘤径2.7mm,接种发根土壤杆菌的株最大瘤径范围为0～11.6mm,平均瘤径3.0mm,根据抗性评价标准判定陕甘山桃群体高抗根癌土壤杆菌和高抗发根土壤杆菌。但陕甘山桃对根癌土壤杆菌和发根土壤杆菌的抗性存在广泛的的株间分离现象,均分离为免疫、高抗、中抗、低抗、感病和易感病6种类型,对根癌土壤杆菌免疫、高抗、中抗、低抗、感病和易感病型植株分别占群体总数的28%、33%、19%、13%、6%和1%,对发根土壤杆菌免疫、高抗、中抗、低抗、感病和易感病型植株分别占群体总数的16%、46%、15%、13%、8%和2%。陕甘山桃是优异的抗根癌病种质资源。
In order to evaluate the resistance of Prunus davidiana var. Potaninii Rehd to root-knot disease, the seedlings of seedlings were used to study the effects of Agrobacterium tumefaciens Conn and rooting by artificial inoculation Resistance of A. rhizogenes Conn. The results showed that the maximum diameter range of the strain from 60 days after inoculation with Agrobacterium tumefaciens was 0-10.1 mm and the average diameter was 2.7 mm. The maximum diameter range of strain inoculated with Agrobacterium rhizogenes ranged from 0 to 11.6 mm , The average tumor diameter 3.0mm, according to the resistance evaluation criteria to determine the population of Shaanxi Gaoshanxia Agrobacterium tumefaciens and highly resistant to Agrobacterium rhizomatosis. However, the resistance of Agrobacterium tumefaciens and Agrobacterium rhizogenes in Shaanxi Ganshan Peach had a wide range of isolations among isolates, which were all separated into six types of immunity, high resistance, moderate resistance, low resistance, susceptible and susceptible diseases. The plants that were immunized with Agrobacterium tumefaciens, highly resistant, moderately resistant, low resistant, susceptible and susceptible belonged to 28%, 33%, 19%, 13%, 6% and 1% Agrobacterium immunization, high resistance, moderate resistance, low resistance, susceptible and susceptible plants accounted for 16%, 46%, 15%, 13%, 8% and 2% of the total population respectively. Shaanxi Gansu Peach is an excellent anti-cancer germplasm resources.