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目的 研究活性维生素D_3对去卵巢骨质疏松大鼠骨质量25(OH)D_3和1,25(OH)_2D_3水平及肾脏VDR mRNA表达的影响,为临床防治骨质疏松提供依据。方法 36只八月龄健康雌性SD大鼠随机分为3组,即模型组(Model),假手术组(Sham),活性维生素D3治疗组(Treatment),每组12只,对照治疗三月后全部处死,用双能X线骨密度仪及生物力学技术评估模型的骨密度及骨质量,采用酶联免疫吸附方法检测血清或组织中25(OH)D3和1,25(OH)_2D_3含量,用FQ-PCR方法检测肾脏组织中VDR mRNA的表达情况。结果 (1)模型大鼠股骨头及粗隆部的BMD及骨生物力学指标(最大载荷和最大压应变)均明显下降(P<0.05);(2)活性维生素D3治疗组与模型组比较,血清、肝脏和肾脏组织中25(OH)D_3和1,25(OH)_2D_3的含量明显提高(P<0.05),与假手术组比较无统计学意义;(3)模型组大鼠肾脏VDR mRNA的表达显著低于活性维生素D3治疗组(P=0.004)。结论本实验结果提示适当补充活性维生素D_3可有效的防治去卵巢大鼠骨量丢失和骨质量下降,为临床活性维生素D应用提供实验依据。
Objective To study the effect of active vitamin D_3 on the bone mass 25 (OH) D_3 and 1,25 (OH) _2D_3 in ovariectomized rats and the expression of VDR mRNA in the kidney of ovariectomized rats, and to provide the basis for clinical prevention and treatment of osteoporosis. Methods Thirty-six healthy female SD rats aged 8 months were randomly divided into three groups: model group, sham operation group and active vitamin D3 group. All of them were sacrificed. The bone mineral density and bone mass of the model were evaluated by dual-energy X-ray absorptiometry and biomechanics. The contents of 25 (OH) D3 and 1,25 (OH) _2D_3 in the serum or tissue were detected by enzyme-linked immunosorbent assay The expression of VDR mRNA in renal tissues was detected by FQ-PCR. Results (1) BMD and biomechanical parameters (maximum load and maximum compressive strain) of femoral head and trochanter in model rats decreased significantly (P <0.05). (2) Compared with model group, The contents of 25 (OH) D_3 and 1,25 (OH) _2D_3 in serum, liver and kidney were significantly increased (P <0.05), and there was no significant difference between the sham operation group and the sham operation group. (3) Was significantly lower than that of the active vitamin D3 group (P = 0.004). Conclusion The results of this experiment suggest that proper supplementation of active vitamin D 3 can effectively prevent bone loss and decrease bone mass in ovariectomized rats, providing an experimental basis for the application of clinically active vitamin D.