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目的应用血清蛋白质组学探索糖尿病慢性肾脏疾病(CKD)致病因子和早期诊断指标。方法将研究对象分为正常对照组(NC)、尿白蛋白/肌酐(UACR)<30 mg/g组、30≤UACR<300 mg/g组及UACR≥300 mg/g组。收集各组空腹血清,去除白蛋白,双向电泳分离,银染。分析差异表达蛋白质点,挖取、酶解后进行质谱分析,与Swiss Prot数据库匹配鉴定。结果各组匹配蛋白点共有2119个,挖取11个差异表达蛋白质点进行质谱分析,成功鉴定其中4个蛋白质点。甘露糖结合凝集素相关丝氨酸蛋白酶2(MASP2)在UACR<30 mg/g组表达增高;前凝血酶原在30≤UACR<300 mg/g组表达增高;血红蛋白α亚单位、免疫球蛋白α1(Igα1)链C区段在UACR≥300 mg/g组表达增高。结论临床血清蛋白质组学可作为CKD诊断有效的研究手段。MASP2、前凝血酶原、血红蛋白α亚单位、Igα1链C区段与CKD的发生发展有关。
Objective To explore the etiological factor and early diagnosis of diabetic chronic kidney disease (CKD) by serum proteomics. Methods The subjects were divided into normal control group (NC), urinary albumin / creatinine (UACR) <30 mg / g group, 30≤UACR <300 mg / g group and UACR≥300 mg / g group. Fasting serum of each group was collected to remove albumin, two-dimensional electrophoresis separation, silver staining. Analysis of differentially expressed protein spots, digging, mass spectrometry analysis after enzymatic hydrolysis, and the Swiss Prot database matching identification. Results A total of 2119 matched protein spots in each group were scored, and 11 differentially expressed protein spots were scored for mass spectrometry analysis, of which 4 protein spots were successfully identified. The expression of mannose-binding lectin-associated serine protease 2 (MASP2) increased in UACR <30 mg / g group; prethrombin expression increased in 30 UACR <300 mg / g group; hemoglobin α subunit, immunoglobulin α1 Igα1) chain C segment in the UACR ≥ 300 mg / g group increased expression. Conclusion Clinical serum proteomics can be used as a diagnostic tool for CKD. MASP2, prothrombin, hemoglobin α subunit, Igα1 chain C segment with the occurrence and development of CKD.