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将30只雄性SD大鼠(体重240~300 g)随机分为3组,分别为对照组(不进行缺氧处理)、低氧组及NK-1R拮抗剂组,建立大鼠缺氧模型,NK-1R拮抗剂组腹腔注射P物质受体(NK-1R)拮抗剂(S3144)进行干预。观察各组大鼠肝组织病理学变化,检测肝功能指标及Tunnel法测肝凋亡细胞百分比,探讨P物质受体(NK-1R)拮抗剂对缺氧大鼠模型肝损伤的影响及其机制。结果显示:(1)缺氧模型的大鼠肝细胞肿胀,排列紊乱,气球样变性较多,并有点状坏死灶,损伤较NK-1R拮抗剂组严重。(2)NK-1R拮抗剂组GGT[(6.750 0±1.669 1)U/]明显低于低氧组[(9.000 0±1.414 2)U/L)](P<0.05);ALT、AST、ALP各组之间比较无明显差异(P>0.05)。(3)NK-1R拮抗剂组肝细胞凋亡百分比[(26.626 3±10.934 8)%]明显低于低氧组[(44.581 4±17.324 5)%](P<0.05)。研究表明:P物质可能与缺氧性肝损伤有关,通过P物质受体(NK-1R)拮抗剂可能减轻缺氧性肝损伤。
Thirty male Sprague-Dawley rats weighing 240-300 g were randomly divided into three groups: control group (without hypoxia), hypoxia group and NK-1R antagonist group. The hypoxia model was established in rats, The NK-1R antagonist group was intraperitoneally injected with the substance P receptor (NK-1R) antagonist (S3144) for intervention. The pathological changes of liver tissue of rats in each group were observed, the indexes of hepatic function and percentage of apoptotic liver cells by Tunnel were detected, and the effect of substance P receptor (NK-1R) antagonist on hepatic injury in hypoxic rat model and its mechanism . The results showed that: (1) Hypoxic rat liver cells swollen, arranged disorder, more balloon degeneration, and a little necrosis, damage than the NK-1R antagonist group. (2) The GGT [(6.750 ± 1.669 1) U / L) in NK-1R antagonist group was significantly lower than that in hypoxia group [(9.000 ± 1.414 2) U / L) There was no significant difference between ALP groups (P> 0.05). (3) The percentage of apoptosis of hepatocytes in NK-1R group [(26.626 3 ± 10.934 8)%] was significantly lower than that in hypoxia group [(44.581 4 ± 17.324 5)%] (P <0.05). Studies have shown that: substance P may be related to hypoxic liver injury, hypoxic liver injury may be alleviated by substance P receptor (NK-1R) antagonist.