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目的:观察人参总皂苷(TG)对野百合碱(MCT)所致大鼠右心室肥厚的影响并探讨其与一氧化氮途径的关系。方法:雄性SD大鼠随机分为:正常对照组、模型组、TG低、中、高剂量组(20,40,60 mg·kg-1·d-1)以及L-精氨酸组(L-arg,200 mg·kg-1·d-1,L-a组);另外,为探讨TG的作用与NO释放的关系,另设TG+L-N(NOS合酶抑制剂NG-硝基-L-精氨酸-甲酯,L-NAME)组和L-a+L-N组。在腹腔注射TG 40 mg·kg-1·d-1或L-arg 200 mg·kg-1·d-1的同时分别灌胃NOS抑制剂L-N 20 mg·kg-1·d-1,各组动物均给药18 d。测定各组大鼠的右心室收缩压(RVSP)、右室肥厚指数(RVHI)、右心重/体重(RVW/BW);透射电镜观察心肌细胞超微结构的改变;硝酸还原法检测心肌组织NO2-/NO3-的含量;Real time RT-PCR检测心肌组织心房利钠因子(ANF)、内皮型一氧化氮合酶(eNOS)mRNA的表达。结果:TG低、中、高剂量和L-arg预防给药均使RVSP,RVSP,RVHI,RV/BW及ANF mRNA表达明显降低(P<0.05),改善细胞线粒体肿胀、变性,L-N能阻止L-arg对上述指标的影响,而同样剂量的L-N并不能影响TG 40 mg·kg-1对上述指标的降低作用;TG 20,40 mg·kg-1不影响eNOS mRNA的表达,但60 mg·kg-1可提高eNOS mRNA的表达。结论:TG能明显改善MCT诱导的大鼠右心室肥厚,其抗心肌肥厚作用部分是通过NO途径实现的。
Objective: To observe the effect of ginseng total saponin (TG) on right ventricular hypertrophy induced by monocrotaline (MCT) in rats and its relationship with nitric oxide pathway. Methods: Male Sprague-Dawley rats were randomly divided into normal control group, model group, TG low, medium and high dose groups (20, 40, and 60 mg · kg -1 · d -1) and L-arginine group -arg, 200 mg · kg-1 · d-1, La group). In addition, TG + LN (NOS synthase inhibitor NG-nitro- L-NAME) group and L-a + LN group. The rats were given intragastric administration of 20 mg · kg-1 · d-1 NOS inhibitor at the same time of intraperitoneal injection of TG 40 mg · kg -1 · d -1 or L-arg 200 mg · kg -1 · d -1 The animals were given 18 days. Right ventricular systolic pressure (RVSP), right ventricular hypertrophy index (RVHI) and RVW / BW were measured in each group. Ultrastructural changes were observed by transmission electron microscopy. NO2 - / NO3-. Real-time RT-PCR was used to detect the expression of atrial natriuretic factor (ANF) and endothelial nitric oxide synthase (eNOS) mRNA. Results: Low, medium and high doses of L-arg could prevent the expression of RVSP, RVSP, RV / BW and ANF mRNA significantly (P <0.05), and improve the mitochondrial swelling and degeneration of cells. -arg on the above indexes, while the same dose of LN did not affect the effect of TG 40 mg · kg-1 on the above indexes; TG 20,40 mg · kg-1 did not affect the expression of eNOS mRNA, but 60 mg · kg- kg-1 can improve eNOS mRNA expression. Conclusion: TG can significantly improve MCT-induced right ventricular hypertrophy in rats, and its anti-hypertrophy effect is partly through the NO pathway.